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Home > TEAMS > TEAM SIBARITA > MICROSCOPES

3D dSTORM

J.B. Sibarita, C. Butler and R. Galland


The 3D dSTORM project is a collaborative project with the company Imagine Optic. It uses a MicAO adaptive optics system, allowing astigmatic-based 3D imaging. We could perform 3D localization over more than a micrometer within the first few micrometers of fixed brain tissue by correcting both system and depth-dependent spherical aberrations [1].


This work was presented as a poster at the Single Molecule Localization Microscopy Symposium in Bordeaux in September and orally at the Adaptive Optics and Wavefront Control in Microscopy and Opthalmology conference in Paris in October.


Furthermore, we hosted a training session in June as part of the Imaging the Cell conference, during which we demonstrated the adaptive optics methods compared to more traditional 3D dSTORM imaging, including an integrated MetaMorph plugin for controlling MicAO, which was developed as part of a collaboration with Imagine Optic.


[1] Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin, Chamma I, Letellier M, Butler C, Lim KH, Gauthereau I, Choquet D, Sibarita JB, Park S, Sainlos M, Thoumine O, Nature Communication, (2016)