Contact: Christelle Breillat - Tel. +33 (0)5 33 51 47 31
Resources in cell culture, part of the Cell Biology core facility of IINS
The cell culture resource is part of the cell biology core facility of IINS. It provides primary hippocampal neurons (mice and rat), organotypic slices and heterologous cell culture (COS-7 cells, HEK, fibroblasts). DNA transfer can be done either by transfection (transfection reagents or electroporation) or viral transduction (lentivirus, AAV, sindbis virus).
Levels of confinement
The cell culture facility is divided into 2 cultures laboratory with a L2 level of confinement, one this L2 lab is also in negative pressure relative to intermediate room.
Equipments for cell culture
These cell culture rooms are equiped with the following:
- 9 laminar flow hoods
- 2 horizontal flow hood for dissections with stereoscope
- 18 CO2 incubators
- 3 inverted microscopes for examination of cell growth, 2 of them are equiped with fluorescence
- refrigerators / freezers
- refrigerated centrifuge, water bath 37°C
- 2 nucleofactor from Lonza
- 2 picospritzers for viral injection in slices
Note: Two laminar flow hoods and two CO2 incubators are dedicated to virus transduction.
The cell culture resource of IINS can help you in setting up your culture system. It can also provide primary neuronal cultures on a contract basis.
BENQUET Sébastien, AI CNRS - T. 05 57 57 57 43
BOUCHET Delphine, IE CNRS - T. 05 57 57 57 47
BREILLAT Christelle, IR CNRS - T. 05 33 51 47 31
DABURON Sophie, AI CNRS - T. 05 57 57 40 91
DURAND Pauline, AI CNRS - T. 05 57 57 57 42
RETAILLEAU Natacha, AI UB - T. 05 57 57 40 94
TESSIER Béatrice, AI CNRS - T. 05 33 51 47 29
VERDIER Emeline, T UB - T. 05 57 57 40 96