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Home > TEAMS > TEAM SIBARITA > TEAM MEMBERS

Makhlad Chahid’s Homepage



Postdoctorate fellowship
LabEx BRAIN
University of Bordeaux
Interdisciplinary Institute for Neurosciences
146 Rue Léo Saignat
33077 Bordeaux
makhlad.chahid at u-bordeaux.fr
Phone: (+33)5 57 57 56 74





Recent News

Our paper called "Multiprotein Printing by Light-Induced Molecular Adsorption" has been accepted for publication in Advanced Materials.



Research interests

Optics, Physics, Instrumentation, Bio-imaging, Microscopy, Super resolution, Compressive Sampling & Inverse problems, Multidimensional imaging.



Research

PhD research:

I achieved my PhD thesis (Dec. 2014) on Compressive Fluorescence Microscopy for biological imaging. This work is an implementation of the recent « Compressive Sampling » (CS) theory that leverages compressibility. Based on a paradigm shift in signal processing our application shows:

  • a unique scheme for 2D imaging using far less measurements than traditional Shannon-Nyquist sampling theory,
  • an extension to Hyperspectral Imaging scheme where multidimensionality introduce high redundancy that greatly increase compression ratio as the signal becomes sparser.

Another part of this work consisted in exploring the possibility to use Compressive Sampling theory for Single Molecule Localization Microscopy (SMLM) at high densities for super resolution microscopy (PALM/STORM). Using this technique, it is possible to drastically reduce the frame stack size by increasing the number of molecules within a region of interest, despite most of molecules are overlapping (because of the point spread function). Thus, one can expect to reconstruct super resolved microscopy images while strongly reducing acquisition time (live imaging).

Former projects:

I have been deeply involved in several projects dedicated to ultra intense laser-matter interactions in the framework of fast ignition (FI) approach to inertial fusion. The lack of experimental data has motivated various studies in the High Energy Density Physics (HEDP), especially in the field of Warm Dense Matter (WDM) where there is a critical need to ensure atomic physics models and simulations tools. I contributed on multiple experiment campaigns where we investigated coupling efficiencies of high-contrast laser irradiation to nanofabricated targets through 2D Kα fluorescence imaging and spectroscopy. These experiments had been performed on both LLNL (CA, USA) and LULI (Paris, France) facilities (respectively PW and TW lasers).



Carrer

Since 2015:Postdoctoral fellowship in the « Quantitative Imaging of the Cell » group at Interdisciplinary Institute for Neuroscience - CNRS UMR 5297 (Bordeaux, France).

2010 - 2014: : PhD in bioimaging with highest honors, supervised by Vincent STUDER (IINS, Univ. Bordeaux, UMR 5297) & Maxime DAHAN (Physico-Chimie, Institut Curie, UMR 168)

University of Bordeaux - Interdisciplinary Institute for Neuroscience - CNRS UMR 5297 (Bordeaux, France).

2007 - 2008: MSc. degree in Physics, with honors, University Bordeaux 1 (Talence, France)



Proficiencies

Technique
Fluorescence microscopy, Super-resolution (SMLM), Structured illumination, Compressive sampling, Spectroscopy, Interferometry.

Optics
Fluorescence microscopy, Structured illumination microscopy, Digital Light Processing, Single pixel imaging, Laser amplification, 2D Kα fluorescence imaging.

Computational

  • MATLAB: Data acquisition, Coding and programming, Algorithm, Image processing, Image analysis, Automation, Simulation and modeling,
  • MicroManager & MetaMorph: Data acquisition, Data analysis,
  • Labview: Data acquisition, Automation,
  • ImageJ: Data analysis and Image processing,
  • Operating systems: Mac OS, Windows and Unix architectures
  • Vector drawing.

Instrumentation
Epi-microscope, Digital Micromirror Device & MEMS, Optical design: dimension design, alignment and optimization, Pahoto-detection: CCD & EMCCD, Photo Multiplier Tube (PMT), photodiode, intensification, streak camera, high throughput laser facilities (amplification, compression).


Publications

Multiprotein Printing by Light-Induced Molecular Adsorption.
Strale, P.-O., Azioune, A., Bugnicourt, G., Lecomte, Y., Chahid, M., & Studer, V.
Advanced Materials 2015.
http://doi.org/10.1002/adma.201504154

Compressive fluorescence microscopy for biological and hyperspectral imaging.
Studer, V., Bobin, J., Chahid, M., Mousavi, H. S., Candes, E., & Dahan, M.
Proceedings of the National Academy of Sciences of the United States of America 2012;E1679-87.
http://doi.org/10.1073/pnas.1119511109

Enhanced isochoric heating from fast electrons produced by high-contrast, relativistic-intensity laser pulses.
Perez, F., Gremillet, L., Koenig, M., Baton, S. D., Audebert, P., Chahid, M., et al.
Physical Review Letters 2010;104(8), 085001.
http://doi.org/10.1103/PhysRevLett.104.085001


Enhanced hot-electron localization and heating in high-contrast ultraintense laser irradiation of microcone targets.
Rassuchine, J., d’Humières, E., Baton, S. D., Guillou, P., Koenig, M., Chahid, M., et al.
Physical Review E 2009;79(3 Pt 2), 036408.
http://doi.org/10.1103/PhysRevE.79.036408


Hyperspectral Fluorescence Microscopy based on Compressive sampling.
Chahid, M., Bobin, J., Mousavi, H. S., Candes, E., Dahan, M. & Studer, V.
Signal Processing with Adaptive Sparse Structured Representations (SPARS) 2013.
EPFL, Lausanne (Switzerland), poster presentation, July 2013

Compressive fluorescence microscopy for biological imaging.
Chahid, M., Bobin, J., Mousavi, H. S., Candes, E., Dahan, M. & Studer, V.
GDR ISIS & STIC SANTE, Telecom Paris, Paris (France), oral presentation, November 2012.

Hyperspectral Fluorescence Microscopy based on Compressive sampling, Computational techniques in Microscopy.
Chahid, M., Bobin, J., Mousavi, H. S., Candes, E., Dahan, M. & Studer, V.
IEEE International Symposium for Biomedical Imaging, poster presentation, Barcelona (Spain), April 2012

Radio interview « Que cherchent-ils ? » in collaboration with Cap Sciences Bordeaux, « Makhlad Chahid is interested in data acquisition optimization in bio-imaging systems with a unique and novel approach by applying Compressed Sensing theory for fluorescence microscopy ».
RCF Bordeaux radio station, May 21th 2012.
http://www.rcf.fr/embed/348933


Single Molecule Localization Microscopy Symposium (SMLMS 15), University of Bordeaux, Bordeaux (France), organization committee team member, August 2015