Supervisory authorities

CNRS


Presentation of the institute

Search




Home > TEAMS > TEAM THOUMINE > RESEARCH AXES > Simulations of protein dynamics

Monte Carlo simulations of protein dynamics


Based on our single particle tracking data on AMPARs, we built a general model based on Monte Carlo computer simulations, integrating the two major mechanisms of AMPAR delivery at synapses, namely surface diffusion and recycling from internal stores (Czöndör et al., PNAS 2012). The main message is that AMPAR delivery at synapses or removal from synapses, is much more efficient if exo/endo-cytosis events occur very close to the synapse, which acts as a trapping element. This model provides new insights on the dynamic regulation of synaptic strength, and was highlighted by a review on these processes (Czöndör and Thoumine, Brain Res Bull 2013). See Video

Since then, we have applied a similar modeling approach to the description of the actin cytoskeleton dynamics in motile neuronal structures, namely growth cones and dendritic spines. In addition to taking into account the fast diffusion of actin monomers, we introduced in the simulations a component describing the slow directed retrograde flow of actin filaments. We were able to nicely fit experimental SPT-PALM and FRAP data obtained from fluorescently-tagged actin in these structures (Garcia et al., PNAS 2015; Chazeau et al., MBoC 2015).