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Pole in vivo - PIV

Monday 6 October 2014, by Marie-Noelle Gouineau


The Pole In Vivo, animal facility for rodents (Mouse and Rat) of the New Aquitaine Broca Center comprise 1400 m2 divided into 3 sectors:

1) The E.O.P.S. Broca facility is dedicated to transgenic animal production area and [*cryopreservation service called CryME for CryoMouseEmbryo*]. This division is coordinated with the central animal facilities (SCA) of Bordeaux University.

2) The [*PIVexpé*], hosts transgenic and wilt type rodents for experiments. This division is coordinated by the Interdisciplinary Institute for Neuroscience (IINS-UMR 5297) and Institute for Neurodegenerative Diseases (IMN-UMR 5293) through shared management.

3) A technical and logistics area supporting the two animal facilities.
The Pôle in Vivo was conceived as a functional tripartite entity.

The capacity of each zone
- 2520 mouse cages OR 1008 rat cages in E.O.P.S.
- 1280 mouse cages and 1000 rat cages in PIVExpé
Animals are in individual ventilated cages with automatic water supply.

[**PIV goals*]

* A rationalized management of the transgenic lines in order to freeze store mouse lines as soon as they are not involved in research projects anymore and avoid the maintenance of live lines when there are no continuous needs. For economic, ethical (Reduce the use of animals – in the 3R ethical rules) and human resource reasons we needed to implement such flexibility. The only way of entry for transgenic lines is embryo transfer for sanitary reasons. This transgenic processing service has been named CryME for Cryo Mouse Embryo. The CryME aims at supporting the whole Bordeaux community and is headed by Pierre Costet.

* The E.O.P.S. will be the only provider of transgenic animals for the experimental area. In a collaborative setting, transfers of lines from one EU to another will go through the transfer of embryos and not following the examination of sanitary controls which are only a snapshot of a health condition. The target status in E.O.P.S is therefore SOPF and SPF in PIVexpé. The cage cleaning facility has been planned for a forward walk flow within the 3 sectors, dirty, clean and sterile with equipment interfacing between sectors (washing cabin, autoclave and SAS H202)

* Automation and robotisation of zootechnical tasks. The cleaning facility is equipped with full-foot equipment and reduces handling (centralized disposal of dirty litter and robot to empty the cages)

* We planned the integration of needs from all research teams to present a maximum of equipments and avoid redundancies.

* 16 gaseous anesthesia workstations for all surgeries, including 3 under PSM for handling class 2 GMOs.

* Devices for behavioral experiments (memory, anxiety, locomotor activity, pain) and electrophysiology experiments on anesthetized or vigilant animals.

* A reflexion for the optimization of the well-being of the animals with a particular care in the choice of equipment of euthanasia with CO2 and anesthesia with isoflurane and equipment for post-surgical recovery with a warmed and enriched in 02 air.

The PIV work flow is based on the principle that "the animal that goes out does not come back in". It is released in annexes in stages 1/2/3 for terminal protocols (dissociated neuron culture, slices in culture, acute slices for electrophysiology, organ harvesting for biochemistry, and molecular biology, electrophysiolgic recording on anesthetized animals followed by euthanasy).

[**Board commitees governing the PIV*]

* A user committee composed of representavies for each research team. This committee ensures the communication on logistics issues, the integration of experimental equipment, the reporting of cryopreservation work, and the diffusion of information from the two EUs.
* The SBEA is managing the surveillance and improvement of animal care and wellness.
At the PIV-EXPE the SBEA is under the responsibility of Audrey Lacquemant from the IINS and while at the EOPS Broca the SBEA is under the responsibility of Mélissa Deshors from the IINS.

* A group is in charge of the monitoring of the use of toxic drugs. Pierre Costet for the EOPS Broca and Evelyne Doudnikoff for the IMN for the PIV-EXPE.

* The competence track for the two EU is provided by Elisabeth Normand who has set up competence’s passports.

[**CHRONIC animal facility outposts*]

An outpost of the facility has been settled next to the IINS laboratorys to allow for advanced chronic "in vivo" imaging protocols for up to several weeks. this outpost is under the responsibility of Christelle Martin.

[*Managers*]

Elisabeth NORMAND, research engineerCNRS
elisabeth.normand@u-bordeaux.fr/Tel 05 33 51 48 24

Christelle MARTIN, research engineerCNRS
Christelle.martin@u-bordeaux.fr/ Tel: + 33 (0) 5 33 51 48 54

Pierre COSTET, research engineer Bordeaux University
pierre.costet@u-bordeaux.fr/ Tel: + 33 (0) 5 33 51 48 24

[*Members*]

Élisabeth NORMAND, research engineer CNRS IINS
SOPF animal facility manager (EOPS Broca)
elisabeth.normand@u-bordeaux.fr/ 05 33 51 48 24

Pierre COSTET, research engineer Bordeaux University (Central animal facilities (SCA)
CryME (Cryo Mouse Embryos) manager
pierre.costet@u-bordeaux.fr/ 05 33 51 48 24

Christelle MARTIN, research engineer CNRS IINS
PIV expé manager
christelle.martin@u-bordeaux.fr/ 05 33 51 48 54

Melissa DESHORS, technician CNRS IINS
SOPF animal facility and CryME
melissa.deshors@u-bordeaux.fr/ 05 33 51 48 55

Anh VERNOY, technician CNRS IMN
SOPF animal facility
melissa.deshors@u-bordeaux.fr/ 05 33 51 48 55

Gregory ARTAXET, technical assistant Bordeaux University (Central animal facilities (SCA)
SOPF animal facility
gregory.artaxet@u-bordeaux.fr/ 05 33 51 48 55

Audrey LACQUEMANT, technician CNRS IINS
PIV expé facility
audrey.lacquemant@u-bordeaux.fr/ 05 33 51 48 55

Guillaume DABEE, technician CNRS IMN
conventional facility
guillaume.dabee@u-bordeaux.fr/ 05 33 51 48 55

Arthur ROBERT, technical assistant Bordeaux University (Central animal facilities (SCA)
Cleaning facility
arthur.robert@u-bordeaux.fr/ 05 33 51 47 75

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