2015-07: Our paper called "3D high- and super-resolution imaging using single-objective SPIM" has been accepted for publication in Nature Methods. It describes a new and innovative method for Selective Plane Illumination Microscopy that uses a single objective for both excitation and detection and which allow for multi-scale 3D imaging and in depth single-molecule localization microscopy.
I obtained my PhD in the field of light-matter interaction in 2008 at the CEA-Grenoble where I developed a robust fluorescence reader for continuous pathogen agent detection using immunoanalyses assays. I then directed my researches toward biology developing different optical and microfabrication methods to address specific biological issues. During a first post-doctorate at the LiPhy (2009-10), I developed a multi-confocal microscope for simultaneous FCS measurements in several points within living cells. I then developed approaches for 2D and 3D patterning of proteins in the CytoMorpho Lab as well as a method that use the self-assembly behaviours of the actin filaments to create 3D electrical connexions for technological applications (2010-12). During a third Post-doc at IINS (2013-2015), I developed a new and innovative light-sheet microscope architecture based on the combination of micro fabricated devices displaying 45° mirrors and a single objective. I am now a researcher in the team Quantitative Imaging of the Cells at IINS where I am developing different optical super-resolution approaches in order to improve the penetration depth, the spectral capabilities and the temporal resolution of single-molecule based super-resolution methods. These improved imaging capabilities are used to better understand the molecular mechanisms that drive the information transmission through the synapses.
- soSPIM: a new tool for 3D high and super-resolution imaging of biological samples.
- Multicolor 3D Single particle tracking using spectrally displaced localization.
Past noteworthy projects:
- I have developed an innovative method that use the self-assembly properties of the actin cytoskeleton of cells to create 3D electrical connexions for micro-electronic applications. Such bio-inspired approaches aim to take advantage of the controlled behaviours of complex biological systems to develop new and alternative technological devices and functions.
- I have developed a 2D dynamic patterning method that use a focalized laser beam to ablate the cell repellent properties of poly-ethylene glycol (PEG) coating. It has been used to dynamically modify the adhesion properties of surfaces surrounding living cells leading to controlled re-organization of the cell cytoskeleton.
Since 2016: Researcher in the Quantitative Imaging of the Cell team at IINS.
2013 - 2015: Post-doctorate in collaboration between Quantitative Imaging of the Cell team at the Interdisciplinary Institute for Neurosciences (IINS, France) and the Biomechanics of Cell-Cell Contact team at the MechanoBiology Institute (MBI, Singapore).
2010 - 2012: Post-doctorate in the CytoMorphoLab at the CEA-Grenoble (France).
2009 - 2010: Post-doctorate in the team Materials, Optics and Instrumental Techniques for the Life Sciances in the Laboratoire Interdisciplinaire de Physique (LiPhy, France).
2008: PhD in Light-matter interaction called: “Development and integration of a field-oriented fluorescence-based array biosensor for continuous pathogen detection” in the Laboratoire d’Imagerie et Systèmes d’Analyses at the Cea-Grenoble (France).
Optic: Optical microscopy (Wide-field, linear and non linear fluorescence, confocal, super-resolution (PALM, STORM, STED, SIM), light sheet microscopy, laser-based patterning methods, Photo-polymerization processes.
Biology: Molecular and cellular biology of the cytoskeleton; Cell culture, fixation, transfection and staining; Drosophila embryo imaging.
Chemistry: surface functionalization and passivation, Metallization processes.
Technology: Micro-fabrication (lithography and soft lithography), Biosensors, knowledge in microelectronic technologies.
Informatics: Office, Image analysis (ImageJ, Matlab, MetaMorph), 3D reconstruction, Programming skills (ImageJ, Matlab, Labview, Visual Basic).
3D Protein dynamics in the cell nucleus.
Singh, A. P., Galland, R., Finch-edmondson, M. L., Grenci, G., Sibarita, J.B., Studer, V., Viasnoff, V., Saunders, T.
Biophysical Journal 2017; 112; 133-142.
3D high- and super-resolution imaging using single-objective SPIM.
Galland, R., Grenci, G., Aravind, A., Viasnoff, V.*, Studer, V.*, Sibarita, J.B.*
Nature Methods 2015; 12 (7); 641-644.
* Contributed equally to this work
Directed actin assembly and motility.
Boujemaa-Patersky, R., Suarez, C., Galland, R. and Blanchoin, L.
Methods in Enzymology. Reconstituting the Cytoskeleton 2014;283-300
Micro-structured surface with integrated micro-mirrors for 3D multi-scale microscopy.
Galland, R., Viasnoff, V., Grenci, G., Sibarita, J.B. and Studer, V.
Patent pending 2013 – WO 2015/030678 A1
Système et méthode de microscopie par éclairage par la tranche.
Galland, R., Viasnoff, V., Sibarita, J.B. and Levecq, X.
Patent pending 2013 – WO 2015/028493 A1
Fabrication of three-dimensional electrical connections by means of directed actin self-organization.
Galland, R., Leduc, P., Guérin, C., Peyrade, L., Blanchoin, L. and Théry, M.
Nature Materials. 2013;12(5);416-421
Method for obtaining three-dimensional actin structures and uses thereof.
Galland, R., Blanchoin, L. and Théry, M.
Patent pending 2012 – WO 2013/117624 A1
Reprogramming cell shape with live nanopatterning.
Vignaud, T., Galland, R., Tseng, Q., Blanchoin, L., Colombelli, J. and Théry, M.
Journal of Cell Science. 2012;125;2134-2140.
Multi-confocal fluorescence correlation spectroscopy.
Galland, R., Gao, J., Kloster, M., Herbomel, G., Destaing, O., Balland, M., Souchier, C., Usson, Y., Derouard, J., Wang, I. and Delon, A.
Frontier in Bioscience. 2011;E3(2);476-488.
Automatic laser alignment for multifocal microscopy using a LCOS SLM and a 32x32 pixel CMOS SPAD array.
Tyndall, D., Walkera, R., Nguyena, K., Galland, R., Gao, J., Wang, I., Kloster, M., Delon, A. and Henderson, R.
SPIE Proceedings Series. 2011;8086;8086S.
Measuring, in Solution, Multiple-Fluorophore Labeling by Combining Fluorescence Correlation Spectroscopy and Photobleaching.
Delon, A., Wang, I., Lambert, E., Mache, S., Mache, R., Derouard, J., Motto-Ros, V. and Galland, R.
Journal of Physical Chemistry. 2010;114(8);2988-2996.
A novel fluorescence-based array biosensor: Principle and application to DNA hybridization assays.
Schultz, E., Galland, R., Du Bouëtiez, D., Flahaut, T., Planat-Chrétien, A., Lesbre, F., Hoang, A., Volland, H. and Perraut, F.
Biosensors and Bioelectronics. 2008;23(7);987-994.
A Robust Fluorescence Multiplex Immunoassay Biosensor Designed for Field Applications.
Schultz, E., Perraut, F., Flahaut, T., Planat-Chrétien, A., Galland, R., Lesbre, F., Hoang, A., Boutal, H. and Volland, H.
Solid-State Sensors, Actuators and Microsystems Conference. 2007.
TRANSDUCERS International. 2007;955-958.