Team leader: Laurent GROC
"All scientific work is a community achievement and not the work of a single rider"
Stephen Jay Gould
Laurent Groc is research director (DR2 CNRS) and Principal Investigator at the CNRS and Université de Bordeaux. His reasearch focuses on the cellular and molecular mechanisms underlying the maturation of glutamate synapses in physiology and neuropsychiatric conditions, such as psychosis. He received his Ph.D. in Neurosciences in 2000 from Wayne State University (Michigan, USA) and Université de Lyon (France). He then joined the Department of Physiology at the University of Goteborg (Sweden) as a postdoctoral fellow to investigate the physiology of developing synapses. In 2004, he was appointed CNRS young investigator (CR2) in the Bordeaux Neurocampus, to continue his investigation on the maturing synapses using an original combination of approaches. He is now head of a team at the Interdisciplinary Institute for Neurosciences. Over the past decade, he acted as associate editors for several journals, scientific officer for the AERES (Paris), and board members for National and International Funding Agencies. He received several prestigious awards, including the CNRS Young Investigator Award (Médaille de Bronze 2008), 2009 Young Investigator Biology Award (French Science Academy), 2015 Prix Foulon (French Science Academy), 2015 Prix Dassault (Fondation FondaMental), and 2016 Human Frontier Science Program Award.
Contact: Laurent Groc, tel. +33 5 33 51 47 02
Our aim is to uncover the cellular and molecular mechanisms underlying the maturation of developing glutamate synapses and their putative dysfunctions in neuropsychiatric disorders, such as psychosis. We mostly focus our attention on the glutamatergic NMDA and monoaminergic receptor signalling and trafficking, exploring the dynamic interplay between these receptors. As these receptors play fundamental role in the plasticity of brain network and synapses, we further explore how hormones (e.g. stress and sexual hormones) and immune-related factors cross-talk with these receptor complexes in the plasma membrane of brain cells. To tackle these challenges, we develop and use different approaches, such as single molecule imaging in brain tissue, super-resolution imaging, FRET-FLIM, molecular biology, biochemistry, electrophysiology, and behavioural tests in rodents. Schematically, our research lines are:
- Surface interaction and dynamics of NMDA and monoamine receptor dynamics: hub for cross-signalling?
- Regulation of the NMDA receptor synaptic organization: toward an integrative view with extracellular (e.g. astrocytic transporters, co-agonists), transmembrane, and intracellular (e.g. scaffold proteins, proteasome) regulators.
- Probing the extracellular space around a single membrane receptor in a live neuron: imaging new nano-object in live brain tissue.
- Neuro-immune interaction in physiology and psychosis: exploring the interplay between neuronal signalling and autoantibodies, human endovirus, and cytokines during psychosis.
Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain.
Godin AG, Varela JA, Gao Z, Danné N, Dupuis JP, Lounis B, Groc L, Cognet L.
Nature Nanotech. 2016. doi: 10.1038/nnano.2016.248.
Targeting neurotransmitter receptors with nanoparticles in vivo allows single-molecule tracking in acute brain slices.
Varela JA, Dupuis JP, Etchepare L, Espana A, Cognet L, Groc L.
Nature Commun. 2016, 14;7:10947. doi: 10.1038/ncomms10947
Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission.
Murphy-Royal C, Dupuis JP, Varela JA, Panatier A, Pinson B, Baufreton J, Groc L*, Oliet SH*. *Share seniority
Nature Neurosci. 2015 Jan 12. doi: 10.1038/nn.3901.
Surface dynamics of GluN2B-NMDA receptors controls plasticity of maturing glutamate synapses.
Dupuis JP, Ladépêche L, Seth H, Bard L, Varela J, Mikasova L, Bouchet D, Rogemond V, Honnorat J, Hanse E, Groc L.
EMBO J. 2014 Apr 16;33(8):842-61.
Single-molecule imaging of the functional crosstalk between surface NMDA and dopamine D1 receptors.
Ladepeche L, Dupuis JP, Bouchet D, Doudnikoff E, Yang L, Campagne Y, Bézard E, Hosy E, Groc L.
Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):18005-10.
Disrupted surface cross-talk between NMDA and Ephrin-B2 receptors in anti-NMDA encephalitis.
Mikasova L, De Rossi P, Bouchet D, Georges F, Rogemond V, Didelot A, Meissirel C, Honnorat J, Groc L.
Brain. 2012 May;135(Pt 5):1606-21.
The stress hormone corticosterone conditions AMPAR surface trafficking and synaptic potentiation.
Groc L, Choquet D, Chaouloff F.
Nat Neurosci. 2008 Aug;11(8):868-70.