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Julien Dupuis’s Homepage



Julien DUPUIS
Inserm Researcher
Interdisciplinary Institute for Neuroscience (IINS)
University of Bordeaux
146 rue Léo Saignat
33076 Bordeaux - France
julien.dupuis at cnrs.fr
Phone: (+33)5 33 51 47 62


Recent News


2018: Check our latest paper called “Differential Nanoscale Topography and Functional Role of GluN2-NMDA Receptor Subtypes at Glutamatergic Synapses” (doi: 10.1016/j.neuron.2018.09.012) describing how the main NMDAR subtypes are organized at the nanoscale at hippocampal glutamatergic synapses, and how this organization shapes synaptic plasticity.


Research


The evolution of neurotransmitter receptor organization at synapses along time and space shapes the efficacy of neurotransmissions, and thereby supports information processing and storage in the brain. My scientific interests are focused on identifying the actors that allow the anchoring of receptors at synapses, investigating their interaction dynamics and understanding their rearrangements during physiological adaptations supporting cognitive functions or pathological processes associated with neuropsychiatric disorders. During my PhD at the Institute for Structural Biology of Grenoble, I studied the molecular mechanisms governing the functional coupling between the subunits of ATP-sensitive potassium channels, and contributed to the design of electrical nanobiosensors based on the artificial coupling of ion channels to G protein-coupled receptors. Fascinated by the brain, I then decided to explore the contribution of physical associations between receptors and their partners to the physiology of neuronal networks. Moving successively to the laboratories of Martin Giurfa (CRCA, Toulouse), Bernard Bioulac and Jérôme Baufreton (IMN, Bordeaux), and Laurent Groc (IINS, Bordeaux), I investigated how the functional and physical interplay between glutamatergic, dopaminergic and cholinergic receptors and their interactors contribute to adjust the strength of synaptic transmissions and to allow memory formation and storage in invertebrates and mammals. I also started exploring how impairments in this interplay may participate in brain dysfunctions. My present research activities are centred on NMDA glutamate receptors (NMDAR) and consist in:

  • exploring how physical interactions between NMDAR and their intracellular, transmembrane and trans-synaptic partners shape NMDAR signalling and neuronal adaptation
  • investigating how impairments in interactions anchoring NMDAR at synapses may contribute to the pathophysiology of neuropsychiatric diseases (e.g. depression, psychosis)

These activities currently involve Alexandra Fernandes (PhD student) and Delphine Bouchet-Tessier (CNRS Engineer).


Career


Since 2014: Inserm Researcher at the Interdisciplinary Institute for Neuroscience in the Development and Adaptation of Neuronal Circuits group (Bordeaux, France).

2012 - 2014: Postdoctoral fellow at the Interdisciplinary Institute for Neuroscience in the Development and Adaptation of Neuronal Circuits group (Bordeaux, France).
2010 - 2012: Postdoctoral fellow at the Institute of Neurodegenerative Diseases in the Dopamine and Neuronal Assemblies group (Bordeaux, France).
2008 - 2010: Postdoctoral fellow at the Research Center on Animal Cognition in the Experience-dependent Plasticity in Insects group (Toulouse, France).
2005 - 2008: PhD in Cell Biology (University Grenoble Alpes) at the Institute for Structural Biology in the Channels groups (Grenoble, France).


Technical Approaches / Proficiencies

- Electrophysiology
- Single particle tracking
- Super-resolution microscopy


Funding


This axis is currently funded by the Brain & Behavior Research Foundation (NARSAD Young Investigator Grant to JD) and the Fondation pour la Recherche Médicale (PhD extension grant to AF).


Publications

Surface trafficking of neurotransmitter receptors: From cultured neurons to intact brain preparations. Dupuis JP, Groc L (2019). Neuropharmacology, 17: 107642.

Differential Nanoscale Topography and Functional Role of GluN2-NMDA Receptor Subtypes at Glutamatergic Synapses. Kellermayer B*, Ferreira JS*, Dupuis J*, Levet F, Grillo-Bosch D, Bard L, Linarès-Loyez J, Bouchet D, Choquet D, Rusakov DA, Bon P, Sibarita JB, Cognet L, Sainlos M, Carvalho AL, Groc L. Neuron. 2018 106-19. (*equal contributions)

Tracking single membrane targets of human autoantibodies using single nanoparticle imaging. Jézéquel J, Dupuis JP, Maingret F, Groc L (2018). Journal of Neuroscience Methods, 304: 76-82.

Dynamic disorganization of synaptic NMDA receptors triggered by autoantibodies from psychotic patients. Jézéquel J, Johansson EM, Dupuis JP, Rogemond V, Gréa H, Kellermayer B, Hamdani N, Le Guen E, Rabu C, Lepleux M, Spatola M, Mathias E, Bouchet D, Ramsey AJ, Yolken RH, Tamouza R, Dalmau J, Honnorat J, Leboyer M, Groc L. Nature Communications 2017 8: 1791.

Single nanoparticle tracking of N-methyl-d-aspartate receptors in cultured and intact brain tissue. Varela JA*, Ferreira JS*, Dupuis JP*, Durand P, Bouchet D, Groc L. Neurophotonics. 2016 3: 041808. (*equal contributions)

Targeting neurotransmitter receptors with nanoparticles in vivo allows single-molecule tracking in acute brain slices. Varela JA, Dupuis JP, Etchepare L, Espana A, Cognet L, Groc L (2016). Nature Communications, 7: 10947.

A critical role for VEGF and VEGFR2 in NMDA receptor synaptic function and fear-related behavior. De Rossi P, Harde E, Dupuis JP, Martin L, Chounlamountri N, Bardin M, Watrin C, Benetollo C, Pernet-Gallay K, Luhmann HJ, Honnorat J, Malleret G, Groc L, Acker-Palmer A, Salin PA, Meissirel C (2016). Molecular Psychiatry, 21: 1768-80.

Surface diffusion of astrocytic glutamate transporters shapes synaptic transmission. Murphy-Royal C, Dupuis JP, Varela JA, Panatier A, Pinson B, Baufreton J, Groc L, Oliet SH (2015). Nature Neuroscience, 18: 219-26.

Surface dynamics of GluN2B-NMDA receptors controls plasticity of maturing glutamate synapses. Dupuis JP*, Ladépêche L*, Seth H, Bard L, Varela JA, Mikasova L, Bouchet D, Rogemond V, Honnorat J, Hanse E, Groc L (2014). European Molecular Biology Organisation Journal, 33: 842-61. (*equal contributions)

Single molecule imaging of the functional crosstalk between surface NMDA and dopamine D1 receptors. Ladépêche L, Dupuis JP, Bouchet D, Doudnikoff E, Yang L, Campagne Y, Bezard E, Hosy E, Groc L (2013). Proceedings of the National Academy of Sciences of the United States of America, 110: 18005-10.

Dopamine-dependent long-term depression at subthalamo-nigral synapses is lost in experimental parkinsonism. Dupuis JP, Feyder M, Miguelez C, Garcia L, Morin S, Choquet D, Hosy E, Bezard E, Fisone G, Bioulac BH, Baufreton J (2013). Journal of Neuroscience, 33: 14331-41.

- Full list of publications available at https://orcid.org/0000-0003-1660-7782 (ORCID)