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Home > TEAMS > TEAM CHOQUET > RESEARCH AXES > Receptor Organization and Synaptic Function

Receptor Organization and Synaptic Function


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Contact: Eric HOSY

Tel. (+33)5 05 33 51 47 30







Scientific Context

The Membrane Trafficking axis is integrated into the Dynamics of Synapse Organization and Function group .

This activity is headed by Eric Hosy (CR1, senior researcher) and currently involves, Hanna Zieger (Post doctoral fellow, ANR), Côme Camus (PhD student, granted by French ministry) and Diogo Soares (PhD student, granted by European ITN). The topic started when application of new high density super-resolution imaging technique reveals that AMPAR are not homogenously distributed inside the synapse but organized in nanodomains of around 80 nm which contained around 25 receptors aligned with pre-synaptic release site. Based on this discovery, the objective is to couple super-resolution imaging technique (U-PAINT, sptPALM, dSTORM) with electrophysiology and modeling to decipher how glutamate receptor organization and mobility impact on the synaptic transmission properties at the basal state, during plasticities and by some pathologies. The different topics mainly studied in the group concern:
  • The development or the improvement of new high density live imaging technics
  • The understanding of the molecular compounds which organize AMPAR inside the synapse
  • The co-organization between AMPAR-NMDAR and mGluR
  • The regulation of AMPAR dynamic organization during synaptic plasticity and pathologies (autism, mental retardation)

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    Trajectory and localization map of AMPA receptor inside synapse with U-PAINT (left image) and dSTORM (right image) super-resolution techniques. Analysis of such experiments revealed that AMPAR are organized in hot spot called nanodomains, containing around 25 receptors. One aim of the group is to see if there is a correlation between one nanodomain and a miniEPSC (see current traces below).
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    Technical approaches

    We use all the various super-resolution imaging technique on living and fixed tissue (U-PAINT, sptPALM, dSTORM, STED). Calcium imaging, electrophysiology (brain slices and neuronal cell culture), synaptic modeling


    Selected publications

    Giannone G*, Hosy E*, Levet F, Constals A, Schulze K, Sobolevsky AI, Rosconi MP, Gouaux E, Tampé R, Choquet D, Cognet L. 2010 Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density. Biophysical Journal.

    Nair D*, Hosy E*, Petersen J.D, Constals A, Giannone G, Choquet D, Sibarita J-B. 2013 Super-resolution imaging reveals that AMPA Receptors inside synapses are dynamically organized in nanodomains regulated by PSD95. Journal of Neuroscience.

    Pougnet J-T, Toulme E, Martinez A, Choquet D, Hosy E, Boué-Grabot E. 2014 ATP P2X receptors downregulate AMPA receptor trafficking and postsynaptic efficacy in hippocampal neurons Neuron.

    Constals A, Penn AC, Compans B, Toulmé E, Phillipat A, Marais S, Retailleau N, Hafner AS, Coussen F, Hosy E* and Choquet D*. 2015 Glutamate Induced AMPA Receptor Desensitization Increases their Mobility and Modulates Short-Term Plasticity Through Unbinding from Stargazin. Neuron

    Schneider R, Hosy E, Kohl J, Klueva J, Choquet D, Thomas U, Voigt A, Heine M. 2015 Mobility of calcium channels in the presynaptic membrane. Neuron Levet F., Hosy E, Kechkar A, Choquet D, Sibarita JB. 2015 SR-Tesseler: a novel segmentation and quantification method for localization-based super-resolution microscopy data using polygons. Nature methods

    Haas K.T., Compans B., Letellier M., Bartol T.M., Grillo-Bosch D., Sejnowski T.J., Sainlos M., Choquet D., Thoumine O., Hosy E. 2018. Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency. Elife. Jullié D., Stoeber M., Sibarita J-B., Zieger H., Bartol T., Arttamangkul S., Sejnowski T., Hosy E., von Zastrow M. 2019. A discrete presynaptic vesicle cycle for neuromodulator receptors. Neuron

    Compans B., Martineau M., Klaassen R., Bartol T., Butler C., Kechkar A., Perrais D., Sejnowski T., Sibarita J-B., Smit A., Choquet D., Hosy E. 2019. NMDAR and ATP -dependent Long-Term Depression trigger distinct molecular reshuffling, impacting differently neuronal responsiveness. BioRXiv


    Former Members

    Audrey Constal (PhD student) - Kalina Haas (PhD student) - Estelle Toulmé (post-doctoral fellow) - Benjamin Compans (PhD student and post-doctoral fellow) - Julia Goncalves (PhD student)