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Salivary Gland Surveillance - Science Immunology, April 2020

Salivary Gland Surveillance - Science Immunology, April 2020



Salivary gland macrophages and tissue-resident CD8+ T cells cooperate for homeostatic organ surveillance


Pathogen sensing in tissues is critical to generating rapid immune responses. Within these tissues, macrophages and resident memory CD8+ T cells (TRM) work together to detect pathogens, and Stolp et al. use intravital imaging of submandibular salivary glands (SMG) to show that TRM follow tissue macrophage topology in a dynamic manner. Macrophage depletion is associated with reduced TRM motility and a diminished clustering in response to inflammatory chemokines. However, although SMG TRM respond to chemoattractants, autonomous motility is also observed and is mediated by friction and insertion of cellular protrusions into microenvironmental gaps. These findings demonstrate that SMG TRM can use different motility modes in proximity to tissue macrophages to patrol the tissue microenvironment.

Legende: Super-resolution shadow imaging (SUSHI) of an immune organ
(Left) Experimental layout of SUSHI of living salivary gland slices. (Middle) SUSHI image for determination of extracellular space. E, epithelium; BV, blood vessel; Scale bars, 10 μm. (Right) Overview of ECS signal with SMG epithelium and CD11c-YFP+ tissue macrophages. Scale bar, 10 μm.


Authors: Bettina Stolp, Flavian Thelen, Xenia Ficht, Lukas M. Altenburger, Nora Ruef, V. V. G. Krishna Inavalli, Philipp Germann, Nicolas Page, Federica Moalli, Andrea Raimondi, Kirsten A. Keyser, S. Morteza Seyed Jafari, Francesca Barone, Matthias S. Dettmer, Doron Merkler, Matteo Iannacone, James Sharpe, Christoph Schlapbach, Oliver T. Fackler, U. Valentin Nägerl and Jens V. Stein

- See the publication on Science Immunology 03 Apr 2020: Vol. 5, Issue 46, eaaz4371 - DOI: 10.1126/sciimmunol.aaz4371

- Contact: Valentin Nägerl, IINS