{"id":500,"date":"2025-09-08T08:36:38","date_gmt":"2025-09-08T06:36:38","guid":{"rendered":""},"modified":"2025-09-08T08:36:38","modified_gmt":"2025-09-08T06:36:38","slug":"thesis-defense-elena-baz-badillo","status":"publish","type":"buni_events","link":"https:\/\/www.iins.u-bordeaux.fr\/en\/events\/thesis-defense-elena-baz-badillo\/","title":{"rendered":"<span>Thesis defense &#8211; <\/span>Elena Baz Badillo"},"content":{"rendered":"<p><strong>Venue : Centre Broca<\/strong><\/p>\n<p>Defense in English<\/p>\n<hr \/>\n<p><strong>Elena Baz<\/strong><\/p>\n<p>Team : <a href=\"\/?p=166552\">Mechanisms of Adaptive Processes in brain circuits (MAP)<\/a><br \/>\nIINS<\/p>\n<h3>Title<\/h3>\n<p>Role of \u03b3-Protocadherins in the molecular encoding of olivo-cerebellar connections<\/p>\n<h3>Abstract<\/h3>\n<p>During development, millions of neurons in the brain must choose their synaptic partners and undergo deep functional and molecular changes to stabilize specific connections. The olivo-cerebellar circuit presents a highly stereotyped development, with synapse selection happening during strict critical periods, making it a perfect model to study these phenomena. Early after birth, multiple climbing fibers (CF) emerging from the inferior olivary nucleus innervate each Purkinje cell (PC) in the cerebellum cortex. Over the three first postnatal weeks, supernumerary CF are pruned until each PC is mono-innervated by a single CF. Beyond the established role of activity in afferent selection, the molecular mechanisms underlying cell-cell interaction and recognition in this circuit remain unknown. The highly diverse family of gamma Protocadherins (\u03b3PCDH) has been recurrently proposed as part of a \u201cmolecular code of connectivity\u201d in the cerebellum, although experimental evidence remains scarce. Here, using PatchSeq we show that during CF elimination, the diversity of \u03b3PCDH isoforms expressed by individual PC gradually decreases. In addition, overexpressing single isoforms to mimic a premature decrease in \u03b3PCDH diversity in single PCs significantly accelerates CF selection in a hindbrain explant model. Furthermore, we detected a higher similarity in \u03b3PCDH repertoire expressed by PCs connected by the same CF, suggesting that \u03b3PCDH expression in PCs is determined by the presynaptic neuron identity. Overall, our results support the role of \u03b3PCDH as part of the cerebellar molecular code of connectivity with a direct effect in CF-to-PC synapse specificity, and a not random \u03b3PCDH isoform expression.<\/p>\n<h3>Keywords<\/h3>\n<p>Cerebellum &#8211; Protocadherins &#8211; Development<\/p>\n<h3>Jury<\/h3>\n<ul>\n<li>Fekrije Selimi (Centre interdisciplinaire de recherche en biologie (CIRB), Coll\u00e8ge de France).<\/li>\n<li>Peter Scheiffele (Biozentrum, University of Basel).<\/li>\n<li>Emilie Pacary (Neurocentre Magendie).<\/li>\n<li>Thesis supervisor: Mathieu Letellier.<\/li>\n<li>Invited member of jury: Alexandre Favereaux.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Role of \u03b3-Protocadherins in the molecular encoding of olivo-cerebellar connections<\/p>\n","protected":false},"template":"","categories":[],"class_list":["post-500","buni_events","type-buni_events","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.iins.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/buni_events\/500","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iins.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/buni_events"}],"about":[{"href":"https:\/\/www.iins.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/types\/buni_events"}],"wp:attachment":[{"href":"https:\/\/www.iins.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/media?parent=500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.iins.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/categories?post=500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}