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Team : CHOQUET

Research Projects

Cytoskeleton and membrane interplay in synaptic organization and transmission (Anna Brachet).

Project Leader(s): Anna Brachet

Contact: Anna BRACHET

Tel. (+33) 5 33 51 47 32

Anna Brachet is a CNRS researcher. She was trained as a cell biologist in the Lab of Benedicte Dargent in Marseille (France). During her post-doc in the lab of Jose A. Esteban in Madrid (Spain), she discovered that LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery (Brachet et al., JCB 2015). In 2017, she joined the team of Daniel Choquet and launched a new research project on the role of spectrin and its interplay with membrane lipids in synaptic function.

Scientific Context

This activity is headed by Anna Brachet (CR, senior researcher) and currently involves Marie-Lise Jobin (post-doctoral fellow), Léa Sarzynski and Théo Dudon (PhD students). Her objective is to study the role of cytoskeleton proteins and their interactions with the membrane in the dynamic regulation of dendritic spine morphology and physiology.
Past and current funding sources include grants from ANR, Bordeaux Neurocampus Seed Projects and the BRAIN Labex.

Selected Publications

A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression.
Brachet A*§, Lario A§, Fernandez-Rodrigo A, Heisler FF, Gutiérrez Y, Lobo C, Kneussel M, Esteban JA*. Cell Report 2021 Aug 3;36(5):109499. doi: 10.1016/j.celrep.2021.109499. *Corresponding authors § These authors contributed equally
 
LTP induction triggers cholesterol redistribution activating Cdc42 and driving AMPA receptor synaptic delivery.
Brachet A, Norwood S, Brouwers JF, Palomar E, Helms JB, Dotti CG and Esteban JA. The Journal of Cell Biology 2015, Mars16;208(6):791-806. (F1000)

Tetrodotoxin-resistant voltage gated sodium channel Nav1.8 constitutively interacts with ankyrin G.
Montersino A, Brachet A, Fache MP, Angles d’Ortoli S, Liu W, Feracci G, Rueda-Boroni F, Castets F and Dargent B. The Journal of Neurochemistry 2014 June 5; 131:33-41.

Determinants of voltage-gated sodium channel clustering in neurons.
Leterrier C, Brachet A, Dargent B and Vacher H. Seminars in Cell and Developmental Biology 2011 Apr;22(2):171-7.

Ankyrin G restricts ion channel diffusion at the axonal initial segment before the establishment of the diffusion barrier.
Brachet A, Leterrier C, Irondelle M, Fache MP, Racine V, Sibarita JB, Choquet D, Dargent B. The Journal of Cell Biology 2010 Oct 18;191(2):383-95.

Protein kinase Casein Kinase 2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G.
Bréchet A, Fache MP, Brachet A, Ferracci G, Baude A, Irondelle M, Pereira S, Leterrier C, Dargent B. The Journal of Cell Biology 2008 Dec 15;183(6):1101-14.
 

Fundings

ANR JCJC, Seed Projects (Bordeaux Neurocampus) and Labex BRAIN

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