Available positions
Team THOUMINE : Postdoc position in Cellular Neuroscience
2023-01-05
We are hiring one highly motivated postdoctoral scientist to investigate the molecular, structural and functional organization of the glutamatergic tripartite synapse. In light of recent evidence that astrocytes are not just a mere "glue" surrounding neurons but actively regulate synaptic connections, we want to investigate the molecular interactions that drive and maintain astrocytic processes in close contact with neurons and how those interactions dynamically regulate the structure and plasticity of synapses. To address this question, we will combine whole patch-clamp recordings with single-cell RNA seq (“patch-seq”), proteomics, electrophysiology, expansion microscopy and live microscopy in the mouse hippocampus, where the role of astrocytes in synaptic plasticity is well established. We will further assess the impact of autistic mutations targeting astrocytic protein candidates that have been so far considered only from a neurocentric point of view.
For this project, the candidate should have expertise in either patch-clamp electrophysiology or microscopy and show strong interest in molecular and cellular neuroscience with a focus on neurotransmission and synaptic plasticity. The project is to be developed in the team Cell Adhesion Molecules in Synapse Assembly at IINS of the Bordeaux Neurocampus. The position has 2 years initial funding from ANR.
Candidates should send a CV, a brief statement of research experience and reference letters to Mathieu Letellier: mathieu.letellier@u-bordeaux.fr
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Team SIBARITA : Job offer: engineer to develop light-sheet microscopy for neurosciences applications
None
Project description
Light Sheet Fluorescence Microscopy technics (LSFM) has demonstrated to be a method of choice for 3D imaging biological samples at various spatial and temporal scales with minimal photo-damaging effects.Several solutions have been developed in the field of neuroscience to image biological samples ranging from fixed whole brains,to single dissociated neurons growing on a coverslip. In this regard, the Interdisciplinary Institute for Neuroscience (IINS)and the Bordeaux Imaging Center (BIC)are equipped with 3complementary LSFMtechniques: (1) an ultramicroscope for whole brain imaging; (2) a Lattice Light Sheet Microscope(LLSM)to image the first layers of brain slices at high spatial resolution; (3) a single objective selective plane illumination microscope (soSPIM)dedicated to 3D cell cultures and in-depth single-molecule localization microscopy (SMLM).
We aim to complete our catalog by implementing a solution based on the Oblique Plane Microscopy (OPM) architecture, which will be dedicated to fast neuronal sample imaging, ie. brain slices, equipped with local photo-manipulation.
Missions
The candidate missions will be:
- to assemble a custom OPM to address specific neurobiological questions,
- to develop the instrument control software and iii) to participate in the testing and optimization of the OPM in close collaboration with neuroscientists.
Candidate profile
We seek for an independent, motivated and enthusiastic candidate, with an interest in neuroscience and a strong expertise in optics and fluorescence microscopy. Strong interest and good skills in programming for instrumentation are required.The candidate will work in an English-speaking environment, in close interactions with the neuroscientists’ team of the Bordeaux’s Neurocampus.
Contact
A 1 year, renewable, engineer position is available in the framework of the French “Grands Programmes de Recherche” BRAIN awarded to the Bordeaux Neurocampus. Applicants should send a CV, a motivation letter and contact details for at least two referees to:
jean-baptiste.sibarita@u-bordeaux.fr; remi.galland@u-bordeaux.fr; mathieu.ducros@u-bordeaux.fr
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