Dynamic Organization and Function of Synapses

Expertise
News
Selected Publications
Members
Alumni & Guests

Team Leader : Daniel Choquet

"Progress in science depends on new techniques, new discoveries and new ideas, probably in that order." Sydney Brenner

Daniel Choquet is a research director at the CNRS. He obtained an engineering degree from Ecole Centrale (Paris, France) in 1984 and completed his PhD in the lab of Henri Korn at the Pasteur Institute (Paris), studying ion channels in lymphocytes. He did a post-doctoral/sabbatical at Duke University (North Carolina, USA) in the laboratory of Michael Sheetz where he studied the regulation of integrin-cytoskeletal linkage by force. He setup his group in Bordeaux (France) at the Institute for Neuroscience and launched an interdisciplinary program on the combination of physiology, cell and chemical biology and high resolution imaging to study the functional role of the dynamic organization and trafficking of neurotransmitter receptors in synaptic transmission. He is now heading the Institute for Interdisciplinary Neuroscience and the Bordeaux Imaging Center core facility. He is also the director of the center of excellence BRAIN. He is a Member of the French Science Academy and has been awarded three consecutive ERC advanced grants. BioSketch (PDF).

You can check here the Personal page of Daniel Choquet.

General objective

The team develops several research topics, combining neuroscience, physics and chemistry in order to unravel the dynamics and nanoscale organization of multimolecular receptor complexes and their functional rôle in glutamatergic synaptic transmission in health and disease. Recently, the team has engaged in a major program to analyze and understand the interplay between AMPA type glutamate receptor nanoscale dynamics, synaptic plasticity and memory formation in the healthy and diseased brain. Our latest developments are a major thrust in combining molecular and physiological studies as well as analyzing neurodevelopmental disorders related to synapse dysfunction.

Project leaders (Click to reach subprojects)


Anna Brachet	Daniel Choquet	Françoise Coussen	Eric Hosy	Matthieu Sainlos

Research Projects

Team Project Summary

Cytoskeleton and membrane interplay in neuronal and astrocytic organization and functions (Anna Brachet).

Chemical Biology and Protein Engineering (Matthieu Sainlos)

Impact of auxiliary proteins on AMPAR transport, trafficking and physiology (Françoise Coussen).

nanoscale excitatory synaptic physiology (Eric Hosy).

Contribution of AMPAR surface trafficking to Short and long term synaptic plasticity (Daniel Choquet)

Synapse dysfunction in neurodevelopmental disorders (Eric Hosy, Daniel Choquet)

Partnerships

Expertise

Super Resolution Microscopy

Slice physiology

Chemical biology, Protein Engineering

Biochemistry

News

Multicolor Spectrin labeling (ML Jobin)

This is a rat hippocampal neuron in culture stained with a series of markers

Nature Neuroscience Review - Choquet D., Sainlos M. and Sibarita J.B.

We review the latest developments for labelling and functionalizing proteins with small localization and functionalized reporters. We present how these molecular tools are combined with the development of a wide variety of imaging methods that break either the diffraction barrier or the tissue penetration depth limits. We put these developments in perspective to emphasize how they will enable step changes in our understanding of the brain.

Daniel Choquet: prix 2022 de l’Academie nationale des sciences, belles lettres et arts de Bordeaux

Academie nationale des sciences, belles lettres et arts de Bordeaux aims to help develop the ideas, work and research of Academicians. Each year, it rewards in particular personalities for their work or their research or for all of their work in the field of science, literature or the arts.

Thus, in 2022, it was Daniel Choquet, CNRS research director, who was awarded "le grand prix 2022 de l’Academie nationale des sciences, belles lettres et arts de Bordeaux"! Indeed, he achieved this distinction thanks to his main scientific achievement: the discovery that neurotransmitter receptors are in constant motion in the neuronal membrane and that the regulation of this traffic profoundly regulates synaptic transmission. Daniel Choquet will receive his award on March 28 from Pierre Hurmic, Mayor of Bordeaux.

Learn more about: Le grand prix 2022 de l’Academie nationale des sciences, belles lettres et arts de Bordeaux

They talk about it too (articles in French): INSB and Bordeaux Neurocampus

Do you want to know more? Nolwenn Cloarec, communication officer: nolwenn.cloarec@u-bordeaux.fr

Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97, eLife

Caroline Bonnet1, Justine Charpentier1, Natacha Retailleau1, Daniel Choquet1,2, Françoise Coussen1*
1University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, France; 2Bordeaux Imaging Center, Bordeaux, France

eLife. 2023-04-20

DOI: https://doi.org/10.7554/eLife.85609

Françoise Coussen, Director of research at the CNRS at IINS worked on AMPAR intracellular transport and directed this work helped by Daniel Choquet. Caroline Bonnet, PhD student, performed the experiments helped by Justine Charpentier who performed all biochemistry experiments and by Natacha Retailleau (molecular biology).

Find the explanations of the scientists of this publication

Identification of the molecular mechanisms regulating the intracellular transport of glutamate receptors: a new pathway for controlling synaptic plasticity

"The modulation of the efficiency of synaptic transmission between neurons is one of the fundamental processes of memory and learning phenomena. This regulation of the strength of synaptic transmission is largely driven by changes in the number of receptors present at the synapse. In this work, the researchers identify a new mechanism for controlling the establishment of receptors at the level of the synapse through the control of their intracellular transport.

Neurotransmitter receptors, and in particular glutamate receptors, are concentrated in synapses in front of neurotransmitter release sites. However, in the process of their biogenesis, these receptors are synthesized at the level of the endoplasmic reticulum, most of the time several hundred microns from the synapses. They must therefore be transported to the synapses. Our previous work had made possible to visualize for the first time the intracellular transport of AMPA-type glutamate receptors, responsible for the majority of the rapid excitatory transmission between neurons. These receptors are transported rapidly (1-2 microns per second) in vesicles circulating on the microtubules using molecular motors. We observed that, surprisingly, this transport was strongly regulated by neuronal activity.

In this new work, we have identified the molecular mechanisms responsible for these regulations. The cytosolic C-terminal domain of the AMPAR GluA1 subunit is specifically associated with two proteins, 4.1 N and SAP97. We analyzed how interactions between GluA1 and 4.1N or SAP97 regulate GluA1 transport and its exocytosis under basal conditions and after induction of synaptic plasticity (LTP). Our results identify differential roles of 4.1N and SAP97 in controlling the different phases of transport and membrane integration of GluA1.

This work opens new perspectives in understanding the molecular mechanisms that control the establishment and maintenance of glutamate receptors at the synapse during synaptic plasticity."

Daniel Choquet is a member of the 2023 Fulbright France

Created in 1946 at the proposal of the eponymous US senator, Fulbright is an international academic mobility program of the US government. The aim of the program is to promote educational and cultural exchanges between France and the United States.

Since its launch, 21,244 students from both countries have benefited from the program:

12,294 French students have gone to the United States of America,

8,950 Americans have come to France.

Daniel Choquet (director of research at the CNRS, director of IINS, the BIC, the cluster of excellence BRAIN_2030 and team leader at IINS) has just been selected as a laureate of the Fulbright France program. He will be spending a 7 month sabbatical stay in Denver in 2024.

Why did you apply to the Fulbright France Program?

“I was getting organized to perform a 7 month sabbatical in Denver in 2022, in the lab of Pr. Mark Dell’Acqua. Given the cost of living in the US, I needed additional funding to support my stay. The Fulbright organization is a very prestigious academic mobility program of the US government and it was thus natural for me to apply, particularly given that there is a specific partnership with our region Nouvelle-Aquitaine.”

What does it mean to you?

“Well, it does mean a lot. First, in searching the Fulbright alumni file, I found out that both my parents were fulbrighters in the 50s while they did post-docs in Princeton, my mother with Albert Einstein. Second, I realized when I obtained this fellowship that when you enter a new family, the family of Fulbrighters, beyond the money, you enter a support network all over the US that will be both very interesting and valuable.”

Will you take this opportunity to discover or develop a research topic?

“Absolutely, the Department of Pharmacology at the University of Colorado in Denver that I’ll join hosts a large number of high profile neurobiologists that develop orginal cell biology approaches.

We will join our forces with Mark Dell’Acqua and Matthew Kennedy to develop new methods and approaches to control receptor trafficking and visualization.”

What do you think this program will bring you, both professionally and personally?

“In addition to the funding that will help me live there, being a Fulbright fellow in the US is really recognized and will help me develop my network and meet many academics in various disciplines. Fulbright fellow also have a mission to be ambassadors of their country, and I certainly intend to introduce to colleagues to some French cuisine specialties!”

#IMadteIt: Marie-Lise Jobin, a post-doctoral researcher starting out as an associate professor

Marie-Lise Jobin is currently a post-doctoral researcher in Daniel Choquet’s "Dynamic organization and function of synapses" team. Interested in the role of the cytoskeleton in the function and structure of dendritic spines, the scientist is supervised by Anna Brachet. Marie-Lise recently obtained a permanent teaching-research position at the Institut National Polytechnique de Bordeaux (Bordeaux INP). Here, she looks back on her career.

What is your background?
"I studied biology and structural biochemistry at the University of Bordeaux. During my Master’s and PhD, I worked in many different laboratories. First, I spent three months working at the University of Guelph in Ontario, Canada. Then, for my PhD, I joined the Institute of Chemistry and Biology of Membranes and Nano-objects (CBMN) in Pessac. On completion of my studies, I spent a year as a post-doctoral fellow at the Nutrition and Integrative Neurobiology Laboratory (Nutrineuro) in Bordeaux. I then spent over three years at the University of Würzburg in Bavaria, Germany. And for the last four years I’ve been at IINS. My post-doctoral adventure will finally come to an end at the end in September 2023, and I’ll be taking up a position as an associate professor in September!"

Why did you join IINS?
"Generally speaking, I’ve always been interested in biomolecular interactions involving cell membranes. I first worked on synthetic membrane models. However, I’ve always wanted to study more physiological systems. So the neuroscience field allows me to work with systems closer to physiology, and to use my highly interdisciplinary skills. [...] Anna Brachet’s field of research on the role of cytoskeletal proteins, in particular spectrins, and their partners, in the regulation, function and mechano-transduction of dendritic spines fit perfectly my research interests. Moreover, IINS is considered one of the most recognized institutes in the field of neuroscience. Above all, IINS is part of a synergistic system, with close relations to the Bordeaux Imaging Center (BIC). It was a unique opportunity to develop my skills in cutting-edge microscopy techniques and gain access to considerable knowledge in this field!"

Can you tell us about your research?
"I’m trying to understand the role of cytoskeletal proteins, beta spectrins, in neurons, and more specifically in dendritic spines, which are the seat of neuronal transmission. My results showed that beta spectrins are co-organised on a nanoscopic scale in dendritic spines and are particularly important for the stability of these structures. Their membrane or cytoskeleton partners are all the more important in maintaining and stabilising these spectrins under the membrane and influence their nano-organisation."

As a woman in science, what difficulties have you encountered?
"My biggest difficulty has always been a lack of self-confidence. But throughout my career I’ve been lucky enough to meet scientists, men and women who have had faith in me, and who have always supported and encouraged me along the way."

Why do women need to be more recognised in the scientific community?
"It seems quite logical to me that gender diversity, or diversity of any kind, leads to better, more creative and innovative science. I can see that things are changing, slowly but surely, in the right direction, and I’m optimistic for the future!"

Any advice for young researcher?
"I’m proud to have got where I am, to have succeeded in obtaining a permanent position in academia, and to have stayed the course despite many years of not knowing what would happen and never giving up. You have to persevere. It’s often the fear of failing that stops us from moving forward, so don’t hesitate, dare to take the plunge and, above all, trust yourself."

2 days of exciting science on synapse biology around the PhD defense of Agata Nowacka

Monday 6th November 2023

Workshop: Virtual Presynaptic Terminal

We invite you to a workshop where a collaborative team from University College London (UCL) and the University of Warwick will introduce a computational modelling framework that enables in silico exploration of the mechanisms of Ca²⁺-driven neurotransmitter release in different synapses.

Morning session 9:00 – 12:30

• Kirill Volynski: Introduction to the modelling framework with examples linking the model and experimental data.

• Yulia Timofeeva: 3D modelling of presynaptic Ca2+ dynamics, covering Ca2+ influx, buffering, and diffusion.

• Chris Norman: Modelling Ca 2+ activation of vesicular release and short-term synaptic plasticity.

• Round table: model expansion across the synaptic cleft.

Afternoon session 14:00 – 17:00

• Yulia Timofeeva and Chris Norman: hands-on training for application of the virtual presynaptic terminal model. Contact the presenters to book a one-to-one training session.

Registration before October 20: https://evento.renater.fr/survey/workshop-virtual-presynaptic-terminal-iyqqwh9v

Tuesday 7th November 2023

Seminar Molecular and cellular mechanisms of synapse physiology

Venue: Centre Broca Nouvelle-Aquitaine (Amphitheater Broca)

Program

9h30 - Maria Passafaro "Unraveling PCDH19-related pathogenic mechanisms in Developmental and Epileptic Encephalopathy 9 (DEE9)"

10h10 - Andrea Barberis 'Spatial determinants for the interactions of glutamatergic and GABAergic synapses in dendrites of hippocampal pyramidal neurons"

10h50 - Kirill Volynski "Synergistic regulation of neurotransmitter release by different synaptotagmin isoforms"

11h30 - Aude Panatier "Astrocytic EphB receptors control NMDAR functions and memory"

14h - PhD defense of Agata Nowacka "Differential contributions of pre- and postsynaptic components in tuning high-frequency short-term synaptic plasticity"

Contact

Kirill Volynski (k.volynski@ucl.ac.uk), UCL Queen Square Institute of Neurology

Chris Norman (c.norman@ucl.ac.uk), UCL Queen Square Institute of Neurology

Yulia Timofeeva (y.timofeeva@warwick.ac.uk), Department of Computer Science, University of Warwick

PhD Project Overview: AMPA Receptor Dynamics in Synaptic Plasticity

Project Title: Unraveling AMPA Receptor Dynamics in Synaptic Plasticity Using Cutting-edge Imaging and Electrophysiology

Introduction

Synapses, the communication hubs of the brain, rely on their molecular composition and organization for effective information processing. AMPA receptors (AMPARs) are crucial in this process as they mediate fast excitatory synaptic transmission, which is essential for learning and memory. This project aims to explore how the mobility and nanoscale organization of AMPARs influence synaptic plasticity and information processing.

Research Objectives

Investigate AMPAR Trafficking:
- Understand how AMPAR mobility and nanoscale organization control synaptic plasticity.
- Study the interaction of AMPARs with auxiliary proteins like TARP, CNIH, and Shisas.
Develop and Apply Advanced Techniques:
- Combine electrophysiology on brain slices with light sheet imaging, optogenetics, and cell biology.
- Utilize innovative tools to control receptor mobility and subunit composition.
Implement High-Resolution Imaging:
- Enhance Light Sheet Fluorescence Microscopy (LSFM) and Lattice Light Sheet Microscopy (LLSM) with adaptive optics for deep tissue imaging.
- Integrate Single Molecule Localization Microscopy (SMLM) techniques like U-PAINT and DNA-PAINT for nanometer-scale resolution imaging of AMPAR subunits.

Methodology

Electrophysiology: Perform recordings on brain slices to analyze synaptic activity and plasticity.
Light Sheet Imaging: Use LSFM and LLSM to visualize synapses in thick samples with minimal phototoxicity.
Optogenetics: Control receptor dynamics using light-sensitive proteins to manipulate signaling pathways.
SMLM Techniques:
- U-PAINT: Track individual AMPAR movements in rat hippocampal cultures.
- DNA-PAINT: Achieve high-resolution imaging of GluA1 and GluA2 subunits in organotypic brain slices.

Innovations and Expected Outcomes

Adaptive Optics Integration: Correct optical aberrations in LLSM for clearer imaging.
Advanced Marking Techniques: Develop robust methods for deep SMLM imaging in brain slices.
Intracellular Signaling Control: Integrate photoswitchable kinases and phosphatases to study receptor dynamics.

Student Opportunities

Hands-on Experience: Gain practical skills in electrophysiology, advanced microscopy, and molecular biology.
Cutting-edge Research: Contribute to groundbreaking studies on synaptic plasticity and receptor dynamics.
Collaborative Environment: Work with a team of experts in neuroscience and cutting-edge imaging technologies.

Join us in exploring the intricate world of synaptic transmission and plasticity, and make a significant impact on our understanding of brain function and diseases.

To apply, please send complete CV, letter of motivation and up to two letters of recommendations: daniel.choquet@u-bordeaux.fr

Selected Publications

Angela M Getz, Mathieu Ducros, Christelle Breillat, Aurélie Lampin-Saint-Amaux, Sophie Daburon, Urielle François, Agata Nowacka, Mónica Fernández-Monreal, Eric Hosy, Frédéric Lanore, Hanna L Zieger, Matthieu Sainlos, Yann Humeau, Daniel Choquet

High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning Science Advances (2022)
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Choquet D., Sainlos M. and Sibarita J.B.

Advanced imaging and labelling methods to decipher brain cell organization and function Nature reviews Neuroscience (2021)
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Tomohisa Hosokawa, Pin-Wu Liu, Qixu Cai, Joana S. Ferreira, Florian Levet, Corey Butler, Jean-Baptiste Sibarita, Daniel Choquet, Laurent Groc, Eric Hosy, Mingjie Zhang and Yasunori Hayashi

CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation Nature Neuroscience (2021)
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Diogo Bessa-Neto, Gerti Beliu, Alexander Kuhlemann, Valeria Pecoraro, Sören Doose, Natacha Retailleau, Nicolas Chevrier, David Perrais, Markus Sauer & Daniel Choquet

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons Nature Communications (2021)
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Laurent Groc & Daniel Choquet

Linking glutamate receptor movements and synapse function Science (2020)
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Julia Goncalves, Tomas M Bartol, Côme Camus, Florian Levet, Ana Paula Menegolla, Terrence J Sejnowski, Jean-Baptiste Sibarita, Michel Vivaudou, Daniel Choquet, Eric Hosy

Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses Proc Natl Acad Sci U S A (2020)
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Tiago Campelo, Elisabete Augusto, Nicolas Chenouard, Aron de Miranda, Vladimir Kouskoff, Come Camus, Daniel Choquet, Frédéric Gambino

AMPAR-Dependent Synaptic Plasticity Initiates Cortical Remapping and Adaptive Behaviors during Sensory Experience Cell Report (2020)
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Florelle Domart, Peter Cloetens, Stéphane Roudeau, Asuncion Carmona, Emeline Verdier, Daniel Choquet, Richard Ortega

Correlating STED and synchrotron XRF nano-imaging unveils cosegregation of metals and cytoskeleton proteins in dendrites eLife (2020)
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Humeau, Y. and Choquet, D.

The next generation of approaches to investigate the link between synaptic plasticity and learning. Nature Neuroscience (2019)
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Charlotte Rimbault, Kashyap Maruthi, Christelle Breillat, Camille Genuer, Sara Crespillo, Virginia Puente-Muñoz, Ingrid Chamma, Isabel Gauthereau, Ségolène Antoine, Coraline Thibaut, Fabienne Wong Jun Tai, Benjamin Dartigues, Dolors Grillo-Bosch, Stéphane Claverol, Christel Poujol, Daniel Choquet, Cameron D Mackereth, Matthieu Sainlos

Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules Nature Communication (2019)
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Emilie Hangen, Fabrice P Cordelières, Jennifer D Petersen, Daniel Choquet, Françoise Coussen

Neuronal Activity and Intracellular Calcium Levels Regulate Intracellular Transport of Newly Synthesized AMPAR Cell Report (2018)
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Penn, A.C., Zhang, C.L., Georges, F., Royer, L., Breillat, C., Hosy, E., Petersen, J.D., Humeau, Y., and Choquet, D.

Hippocampal LTP and contextual learning require surface diffusion of AMPA receptors Nature (2017)
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Audrey Constals, Andrew C Penn, Benjamin Compans, Estelle Toulmé, Amandine Phillipat, Sébastien Marais, Natacha Retailleau, Anne-Sophie Hafner, Françoise Coussen, Eric Hosy, Daniel Choquet

Glutamate-induced AMPA receptor desensitization increases their mobility and modulates short-term plasticity through unbinding from Stargazin Neuron (2015)
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Anne-Sophie Hafner, Andrew C Penn, Dolors Grillo-Bosch, Natacha Retailleau, Christel Poujol, Amandine Philippat, Françoise Coussen, Matthieu Sainlos, Patricio Opazo, Daniel Choquet

Lengthening of the Stargazin Cytoplasmic Tail Increases Synaptic Transmission by Promoting Interaction to Deeper Domains of PSD-95 Neuron (2015)
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Deepak Nair, Eric Hosy, Jennifer D Petersen, Audrey Constals, Gregory Giannone, Daniel Choquet, Jean-Baptiste Sibarita

Super-resolution imaging reveals that AMPA receptors inside synapses are dynamically organized in nanodomains regulated by PSD95 Journal of Neuroscience (2013)
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Heine, M., Groc, L., Frischknecht, R., Beique, J.C., Lounis, B., Rumbaugh, G., Huganir, R.L., Cognet, L., and Choquet, D.

Surface mobility of postsynaptic AMPARs tunes synaptic transmission Science (2008)
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Aren J Borgdorff and Daniel Choquet

Regulation of AMPA receptor lateral movements Nature (2002)
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Members

« Researcher »

BRACHET Anna	Researcher	anna.brachet@u-bordeaux.fr	+33533514732
CHOQUET Daniel	Researcher	daniel.choquet@u-bordeaux.fr	+33533514715
COUSSEN Francoise	Researcher	francoise.coussen-choquet@u-bordeaux.fr	+33533514734
HOSY Eric	Researcher	eric.hosy@u-bordeaux.fr	+33533514730
SAINLOS Matthieu	Researcher	matthieu.sainlos@u-bordeaux.fr	+33533514731

« Technical Staff »

BADRUNA Louise	Technical staff	louise.badruna@u-bordeaux.fr	+33533514700
BELZANNE Pauline	Technical staff	pauline.belzanne@u-bordeaux.fr	+33533514700
BREILLAT Christelle	Technical staff	christelle.breillat@u-bordeaux.fr	+33533514731
CHEVRIER Nicolas	Technical staff	nicolas.chevrier@u-bordeaux.fr	+33533514733
Daburon Sophie	Technical staff	sophie.daburon@u-bordeaux.fr	+33533514732

« Postdoc »

CORNEBOIS Anaïs	Postdoc	anais.cornebois@u-bordeaux.fr	+33533514700
DOMART Florelle	Postdoc	florelle.domart@u-bordeaux.fr	+33533514700
IGNACZ Attila	Postdoc	attila.ignacz@u-bordeaux.fr	+33533514700
STAJANO Daniele	Postdoc	daniele.stajano@u-bordeaux.fr	+33533514700

« PhD student »

CHANDRA Meera	PhD student	meera.meera-chandra@u-bordeaux.fr	+33533514700
CHARBONNIER Tatiana	PhD student	tatiana.charbonnier@u-bordeaux.fr	+33533514700
CUENOT Chloé	PhD student	chloe.cuenot@u-bordeaux.fr	+33533514700
DARRIBERE Manon	PhD student	manon.darribere@u-bordeaux.fr	+33533514700
GALIZIA Chiara	PhD student	chiara.galizia@u-bordeaux.fr	+33533514700
LEVAL Léa	PhD student	lea.leval@u-bordeaux.fr	+33533514700
PESSOA Emma	PhD student	emma.pessoa@u-bordeaux.fr	+33533514700
SARZYNSKI Léa	PhD student	lea.sarzynski@u-bordeaux.fr	+33533514700
VILLICANA-MUÑOZ Viviana	PhD student	viviana.villicana-munoz@u-bordeaux.fr	+33533514700

« Alumni & Guests »

Former group members, follow-up last known position

Aren borgdorff - 1997-2000, Industry
Arnauld Sergé - 1997-2001, Assistant professor Marseille
Marianne Renner - 2004-2006, Professor, Paris
Caroline Dequidt - 2004-2007, Industry
Cécile Bats - 2004-2007, Post-doc, London
Martin Heine - 2003-2007, Junior group leader, Magdeburg
Enrica Petrini - 2005-2008, Post-doc, Genoa
Cezar Tigaret - 2006-2009, Lecturer, Bristol
Helge Ewers - 2007-2009, Group leader, Berlin
Arnaud Frouin - 2007-2010, Labmanager, NYC
Leandro Royer - 2009-2012, Post-doc, Boston
Patricio Opazo - 2008-2013, Group leader, Brisbane
Jary Delgado - 2009-2013, Post-doc, Chicago
Deepak Nair - 2009-2013, Group leader, Bengalor
Damien Jullié - 2009-2013, Post-doc, San Francisco
Audrey Constal - 2010-2013, Teacher
Axel Athane - 2011-2013, Industry
Dolors Grillo, 2011-2013, Post-doc, Spain
Anne-Sophie Hafner - 2010-2014, Post-doc, Frankfurt
Isabelle Gautherau - 2012-2014, Industry
Amandine Philippat - 2013-2015, Industry
Andrew Penn - 2010-2015, Junior group leader, Sussex
Hongyu Zhang - 2010-2015, Post-doc, University of Bergen
Estelle Toulme - 2013-2015, Post-doc, Berlin
Jennifer Petersen - 2009-2016, Post-doc, NIH
Yulia Krapivkina - 2013-2016, Industry
Ngoc Van Thi Nhu - 2013-2016, Engineer, Montpellier
Emilie Hangen - 2014-2017
Ségolène Antoine - 2015-2017, French Industry
Sara Crespillo - 2015-2017, Post-doc, Cambridge
Célia Michel - 2014-2017, French Industry
Benjamin Compans - 2014-2018, Post-doc, London
Lucile Pret - 2018, Engineer, London
Julia Goncalves – 2015 – 2018, Engineer, Bordeaux Neurocampus
Murielle Fevre - 2017-2018
Camille Genuer - 2017-2018, Engineer, French Industry
Carla Montecinos - 2013-2018, post-doc Bordeaux
Charlotte Rimbault – 2014-2019, Post-doc, Copenhagen
Magalie Martineau – 2015-2019, Post-doc, Paris
Léa Claverie – 2015 - 2019, UK
Florelle Domart - 2016-2019, Post-doc Goettingen
David Perrais - 2011-2020, Group Leader
Sylvia Sposini - 2018-2020, Post-doc, Bordeaux
Lou Bouit - 2018-2020, Engineer, Bordeaux
Côme Camus - 2018-2021, Medical Intern, Bordeaux
Caroline Bonnet - 2017-2021
Inès Gonzalès-Calvo - 2019-2021
Valeria Pecoraro - 2019-2021
Marie-Lise Jobin, 2019-2023 Maîtresse de Conférences (INP, Bordeaux)

Guests

Raffaella Adami, Pisa - January, June 2001
Andres Villu Maricq, Utah - November 2003
Radhika Reddy, Worley lab - June 2003
Michael Ehlers, Duke University - March, September 2006
Renatto Frischknecht, Gundelfinger lab - April 2006, September 2006
Laura Andreae, Fine lab - April 2006, November 2006
Anna Carbone, Plested Lab - January, April 2012
Beulah Leitch, Otago University - October 2013
Andrew Plested, FMP Berlin - October 2015, February 2016
Nikolaj Riis Christensen, Copenhagen University - June-December 2017
Anne Brunet, Stanford University - April-July 2019
Pin Wu Liu, Kyoto University Graduate School of Medicine - January 2020
Boram Lee, Johannes W. Hell UCDavis - January-June 2020